The 71-year-old legendary singer joined the social media site as "Ms. Ross" at @dianaross Wednesday, and declared she is "loving" the experience in her first tweet.
Ross immediately followed her five children, Rhonda Ross, Tracee Ross, Chudney Ross, Ross Naes and Evan Ross, and had over 18,000 followers as of Thursday morning.
Ross came to fame with Motown girl group The Supremes in the 1960s, and subsequently pursued a solo career. She last released I Love You in 2006, and is known for such films as Lady Sings the Blues (1972) and The Wiz (1978).
Ross is mother to Rhonda by Motown founder Berry Gordy, Tracee and Chudney by first husband Robert Ellis Silberstein and Ross and Evan by late second husband Arne Naess, Jr. Evan Ross wed singer Ashlee Simpson in 2014, and the couple are expecting their first child.